Human origin determination is an important aspect of blood grouping analysis in forensic science laboratories. In the present study, protein extractants like gel buffer, ammonia and saline employed for origin determination were evaluated and compared qualitatively and quantitatively for their role in the extraction of proteins from dried blood stained materials of human origin at regular time intervals.
NIH Funded Articles
- Objectively Coding Intervention Fidelity During A Phone-Based Obesity Prevention Study
- Molecular Mechanism Linking BRCA1 Dysfunction to High Grade Serous Epithelial Ovarian Cancers with Peritoneal Permeability and Ascites
- Acculturation and Subclinical Atherosclerosis among U.S. South Asians: Findings from the MASALA study
- Wake-up Strokes Are Similar to Known-Onset Morning Strokes in Severity and Outcome
- Predictors of Obstructive Sleep Apnea Risk among Blacks with Metabolic Syndrome
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Quantitative Analysis of Candida Cell Wall Components by Flow Cytometry with Triple-Fluorescence Staining
Detailed analysis of dynamic fungal cell wall components is crucial to our understanding of fungal systematics and the biology and physiology of fungal growth. In fungal pathogens this is of particular importance in examining their response to stress. However, current methodologies do not permit fast and accurate or quantitative analysis of cell wall carbohydrate components. Here, we provide a novel method permitting simultaneous quantitative analysis of the major cell wall components of Candida species relying on triple-staining with fluorescent labeling of chitin, β-glucans and mannans.
In many species, male social rank has been found to correlate with testosterone concentration ([TEST]), with more dominant males having higher circulating [TEST].
Adrenal Incidentalomas - From Diagnosis to Follow-Up: Endocrine and Imaging Features at Diagnosis and Over Time
Adrenal incidentalomas (AIs) are frequent and although most of them are asymptomatic and benign, some do need treatment. The AIs follow-up has to be as sharp as to avoid missing pathology and to avoid harm and anxiety induced by the follow-up itself. It is, thus, a delicate medical condition for endocrinologists worldwide.